Liquid chromatographic determination of sulfadiazine in salmon by postcolumn derivatization and fluorescence detection
Gehring, T.A.; Rushing, L.G.; Thompson, H.C.
Journal - Association of Official Analytical Chemists 78(5): 1161-1164
1995
ISSN/ISBN: 0004-5756 PMID: 7549531 Document Number: 448460
A reversed-phase (ODS-2) liquid chromatographic method was developed to determine low nanogram-per-gram levels of sulfadiazine (SDZ) in salmon muscle tissue. SDZ was extracted with acetonitrile-aqueous 2% acetic acid (pH 3.0), partitioned into methylene chloride, and cleaned up by using a strong-cation-exchange, solid-phase extraction cartridge. SDZ was derivatized postcolumn with fluorescamine and detected by fluorescence. The limit of detection was 0.2 ng SDZ/g tissue. Recoveries from coho salmon tissue fortified with 1, 5, 10, and 20 ng SDZ/g tissue averaged 84.5, 85.0, 83.6, and 83.9%, respectively; recoveries from Atlantic salmon tissue fortified with 10 ng SDZ/g tissue averaged 82.6%.