Precolumn fluorescence derivatization of the antagonist [Arg6,D-Trp7,9,MePhe8]-substance P[6-11] with benzoin in high-performance liquid chromatography and selective detection of arginine-containing fragments in its degradation products
Cui, H.; Reubsaet, J.L.; Bult, A.
Journal of Chromatography A 736(1-2): 91-96
1996
ISSN/ISBN: 0021-9673 PMID: 8673253 Document Number: 458302
Precolumn fluorescence derivatization for the determination of the antagonist (Arg-6,D-Trp-7,9,MePhe-8)-Substance P(6-11) (antagonist G) using benzoin in HPLC was studied. Under the conditions chosen (0.067 M NaOH, heating at 100 degree C for 10 s), a good yield of fluorescent derivatives was obtained and no methodology-related degradation occurred. The detection limit of antagonist G was 0.21 nmol/ml. The method has been applied to the selective and sensitive detection of arginine-containing fragments in degradation products of antagonist G.