High-performance liquid chromatography determination of histamine in nasal mucosa of guinea pig after post-column derivatization
Huang, X.H.; Chen, B.M.; Liang, S.X.; Deng, F.L.
Hunan Yi Ke da Xue Xue Bao 25(3): 294-296
2000
ISSN/ISBN: 1000-5625 PMID: 12212174 Document Number: 517817
A rapid and simple method for determining histamine by post-column derivatization liquid chromatography with fluorescent detection was described. SCX weakly acidic cation exchange column was used as an analytical column. Histamine was eluted at 13 min 12 s by 40 mmol.L-1 of trisodium citrate (pH5.50) at flow rate of 1.0 ml.min-1. The recoveries of histamine ranged from 1.0 mumol.L-1 to 100 mumol.L-1 were above 92%. The detection limit for histamine was 50 nmol.L-1 and the linear range for histamine was 50 nmol.L(-1)-500 mumol.L-1.