Measurement of monoamine transmitters in the brains of ovariectomized mice using high performance liquid chromatography with fluorescence detection and gradient flow rate elution
Zhang, L.; Lu, Y.; Deng, T.; Zheng, X.
Se Pu 23(1): 76-78
2005
ISSN/ISBN: 1000-8713 PMID: 15881372 Document Number: 594985
A method for the determination of norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in brains of ovariectomized mice was established by using high performance liquid chromatograph with a fluorescence detector, a C18 column and a gradient flow rate delivered by a single pump. The mobile phase was a mixture of 0.1 mol/L KH2PO4 and CH3OH (9/1, v/v). Detection limits of the method were determined to be lower than 10 nmol/L, absolute recoveries were above 80% and the relative standard deviations (RSDs) of intra-day were all within 3.03%. The method has the advantages of high sensitivity and of simple sample preparation. Short analysis time and better separation of samples were also obtained.