Determination of ochratoxin A in animal feed and cereal grains by liquid chromatography with fluorescence detection
Cohen, H.; Lapointe, M.
Journal - Association of Official Analytical Chemists 69(6): 957-959
1986
ISSN/ISBN: 0004-5756 PMID: 3804948 Document Number: 286045
A liquid chromatographic (LC) method is described for determination of ochratoxin A in animal feeds and cereal grains. Samples are initially extracted with chloroform-ethanol (8 + 2) and 5% acetic acid in water. Extracts are purified using a silica gel cartridge followed by a cyano cartridge. The samples are evaporated, diluted to a known volume, and analyzed using a 10 cm column of 3 .mu.m C18 and a fluorescence detector. The method was applied to a variety of animal feeds and cereal grains at levels of 1.0-0.005 ppm added ochratoxin A. The overall recovery was 90.6% .+-. 3.6.