Study of conformational transitions in poly (A) using the buffer capacity method
Zarudnaia, M.I.
Molekuliarnaia Biologiia 32(3): 508-514
1998
ISSN/ISBN: 0026-8984 PMID: 9720076 Document Number: 494713
Curves of proton buffer capacity of poly(A) were obtained; their expansion to basic functions corresponding to ionization of varying type groups was carried out. It was found, that the proton buffer capacity curves of poly(A) had three sharp peaks. The first two peaks were involved in the formation of A- and B-forms of poly(A). It was suggested that the third peak reflected the conformational change of the B-form. A diagram of the distribution of individual forms of poly(A) as a function of pH was presented. Values of pK of three conformational transitions in poly(A) were calculated for various temperatures and ionic strengths on the basis of data from the literature.