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Study of conformational transitions in myosin using 2 types of spin label

Ruuge, E.K.; Medvedeva, N.V.; Vilenkina, M.N.; Bliumenfel'd, L.A.

Biofizika 21(3): 409-412

1976

ISSN/ISBN: 0006-3029
PMID: 183836
Document Number: 101121
It is shown that three types of ESR spectra of myosin labeled with both acetate and acetamide spin labels are distinguished. These three types are the spectrum of initial preparation, myosin--ADP complex spectrum and the spectrum recorded during ATP hydrolysis. The ESR spectra of myosin labeled with acetate spin label are more sensitive to conformational changes of myosin induced by complex formation and temperature.

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