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Fluorescent probe study of temperature-dependent conformational transitions in proteins

Nikushkin, E.V.; Klebanov, G.I.

Biofizika 21(4): 619-623

1976

ISSN/ISBN: 0006-3029
PMID: 1009142
Document Number: 98747
Temperature-dependent conformational features of human serum (SAH) and egg albumins were studied by fluorescent probes such as 3-metoxybenzantron (MBA), dimethylaminocholkon (DMC) and 1-anilononaphtalene-8-sulfonate (ANS) in temperature range of 4--68 degrees C. It was found that these proteins undergo the conformational transitions accompanied by the changes of the quantrum yield and maximum position of fluorescence of MBA, DMA and ANS at 12--16 degrees C, 16--23 degrees C, 40--62 degrees C for SAH and at 5--25 degrees C, 46--64 degrees C for egg albumin.

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