Study of fusion of bacteriophage f2 double-stranded RNA, poly (A) .poly (U) , and poly (G) .poly (C) in the presence of tetraethylammonium bromide
Permogorov, V.I.; Tiaglov, B.V.; Minaev, V.E.
Molekuliarnaia Biologiia 14(5): 1013-1018
1980
ISSN/ISBN: 0026-8984 PMID: 7421813 Document Number: 163120
The data on the dependence of the melting curve parameters of double-stranded RNA (replicative form of RNA of f2 bacteriophage) poly(A) times poly(U) and poly(G) times poly(C) on the concentration of (C2H5)4NBr were obtained. The RNA melting range width is shown to pass through the minimum value T =2.1+/-0.1degrees at the point of inversion of relative stability of GC and AU pairs that corresponds to 4.0+/-0.1 M concentration of (C2H5)4NBr. Using the melting temperatures of poly(A) times poly(U) and poly(G) times poly(C) the rependence of Tgc-Tau parameter on (C2H5)4NBr concentration was shown. It was concluded from these data that the effect of the double-stranded RNA stacking heterogeneity was negligible in the 0-3 M range of (C2H5)4NBr concentration. Melting curves of RNA were obtained at various values of Tgc-Tau parameter. It was shown that the profile of fine structure of melting curves depends on the value of Tgc-Tau parameter.