Isolation of alpha-latrotoxin from karakurt spider venom using monoclonal antibodies
Pashkov, V.N.; Kovalevskaia, G.I.; Krasnoperov, V.G.; Bulgakov, O.V.
Bioorganicheskaia Khimiia 15(9): 1281-1283
1989
ISSN/ISBN: 0132-3423 PMID: 2590256 Document Number: 339862
A method of alpha-latrotoxin (LT) isolation from the venom of Latrodectus mactans tredecimguttatus by means of immunoaffinity chromatography on sepharose conjugated with monoclonal antibodies against LT has been developed. This one-step, high-yield, relatively simple and rapid procedure yields active LT for structural and functional studies of its receptor.
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