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The isolation and characteristics of monoclonal antibodies to the structural proteins of Coxsackie B5 virus

Boss, A.; Piirsoo, A.

Voprosy Virusologii 39(2): 80-82

1994

ISSN/ISBN: 0507-4088
PMID: 8017060
Document Number: 434754
Five hybridomas secreting monoclonal antibodies (MCA) to Coxsackie B5 virus were generated by fusion of Sp2/0 myeloma cells with BALB/c mouse immune splenocytes. Most MCA belonged to the IgG and one to the IgM class. Out of 4 MCA binding the structural protein VP2, two MCA showed type specificity in neutralization test and cross-reactivity with other enteroviruses, one MCA reacted with all enteroviruses tested, and the one showing type specificity among Coxsackie B group "recognized" an epitope on poliovirus type 1 as well. The MCA belonging to VP4 proved to be Coxsackie B group specific. It was assumed that MCA could be used for studies of the antigenic structure and serodifferentiation of Coxsackie B5 virus.

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