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Production and study of hybridomas, producing monoclonal antibodies to the structural glycoprotein of Marburg virus

Ruchko, S.V.; Lebedev, V.N.; Pashchenko, I.I.; Borisevich, G.V.; Semenova, I.S.; Khamitov, R.A.; Maksimov, V.A.; Firsova, I.V.; Petrovskiĭ, A.V.

Voprosy Virusologii 46(6): 21-24

2001

ISSN/ISBN: 0507-4088
PMID: 11785382
Document Number: 532449
Hybridomas producing monoclonal antibodies (MAb) to Marburg virus glycoprotein were prepared by splicing of mouse myeloma cells (NSO and X.63-Ag6.653 strains) and splenocytes of immunized BALB/c mice. Cultural and MAb-producing properties of hybridomas were studied. The production of MAb during serial passages 15-30 was confirmed. MAb titers in culture fluids were 1:4096-1:8152, in immune ascitic fluids of BALB/c mice 1:409,600-1:638,400. The possibility of using MAbs as a component of ELISA test system was demonstrated. The sensitivity of ELISA-MAb method for Marburg virus was 1 x 10(3) PFU/mu.

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