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Storage and reconstruction of hybridomas producing monoclonal antibodies against viral antigens

Malakhova, I.V.; Zorin, E.V.; Novokhatskiĭ, A.S.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 3: 41-44

1985

ISSN/ISBN: 0208-0613
PMID: 3842746
Document Number: 251344
The conditions were optimized for freezing storage, restoring and further cultivation of hybridoma cells producing antibodies to viral antigens. The effect of density of cellular suspension frozen,concentration of calf embryo serum in cryoprotected medium and mild conditions of the restoring of hybridoma were studied. To restore deeply frozen hybridoma 24 hole plastic panels with a layer of feeding cells of the HEPES and insulin containing medium were used. The fulfilling of these requirements makes possible restoration of intact antibody-producing hybridoma from 10(2)-10(3) frozen cells.

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