Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting

Faerman, A.I.; Chervonskiĭ, A.V.; Chipysheva, T.A.; Gel'shteĭn, V.I.; Koliada, A.I.; Faerman, A.I; Chervonskii, A.V; Chipysheva, T.A; Gel&apo; shtein, V.I; Koliada, A.I

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Isolation of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting

Faerman, A.I.; Chervonskiĭ, A.V.; Chipysheva, T.A.; Gel'shteĭn, V.I.; Koliada, A.I.

Biulleten' Eksperimental'noi Biologii i Meditsiny 103(5): 631-634

1987

ISSN/ISBN: 0365-9615

PMID: 3297191

Document Number: 298782

Monoclonal anti-peroxidase antibodies (McAb) were generated by means of hybridization of BALB/c immune splenocytes with X-63.653 cells. Peroxidase-antiperoxidase (PAP) staining of fibroblast cultures with murine McAb against fibronectin was used for the detection of positive cultures. McAb AP-FC-2B4 were found to be highly efficient in different immunoenzyme tests, e.g., immunochemistry and immunoblotting. PAP method was used for histological diagnosis of invasive human breast cancer.