Inhibition of horseradish peroxidase by N-ethylamide of o-sulfobenzoylacetic acid
Rogozhin, V.V.; Kutuzova, G.D.; Ugarova, N.N.
Bioorganicheskaia Khimiia 26(2): 156-160
2000
ISSN/ISBN: 0132-3423 PMID: 10808412 Document Number: 524690
The carboxylic groups of horseradish peroxidase were modified by 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate by the Koshland method. The catalytic properties of the native and modified peroxidase were studied in the presence of N-ethylamide of o-sulfobenzoylacetic acid (EASBA) at pH 5.0-7.5. In the oxidation of o-dianisidine, EASBA is a competitive inhibitor of the carbidiimide-modified peroxidase, and it increases both K(m) and Vm in the case of the native enzyme. These data show that at least one of the carboxylic groups modified with carbodiimide is located at the area of the peroxidase active site.