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Monoclonal antibodies against horseradish peroxidase

Viklický, V.; Dráber, P.; Macků, B.; Dráberová, E.; Rozprimová, L.

Folia Biologica 30(6): 377-382

1984

ISSN/ISBN: 0015-5500
PMID: 6394370
Document Number: 231290
Four hybridomas making monoclonal antibodies against horseradish peroxidase have been established. Two of them are IgM and two are IgG1 with kappa light chains. Antibody HP-03 has been selected as the most suitable one for preparation of the peroxidase-anti-peroxidase complex. Furthermore, optimal conditions for construction and applications of the PAP complex in immunocytochemical and immunohistochemical methods are shown.

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