Oxidation of phenols by horseradish peroxidase and lactoperoxidase compound II--kinetic considerations
Zahida, M.S.; Deva, W.; Peerzada, G.M.; Behere, D.V.
Indian Journal of Biochemistry and Biophysics 35(6): 353-357
1998
ISSN/ISBN: 0301-1208 PMID: 10412229 Document Number: 496724
Oxidation of para substituted phenols by horseradish peroxidase compound II (HRP-II) and lactoperoxidase compound II (LPO-II) were studied using stopped flow technique. Apparent second order rate constants (kapp) of the reactions were determined. The kinetics of oxidation of phenols by HRP-II and LPO-II have been compared with the oxidation potentials of the substrates. Reorganization energies of electron-transfer of phenols to the enzymes were estimated from the variation of second order rate constants with the thermodynamic driving force.