The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products
Pugach, A.V.; Zverev, V.V.; Pille, E.R.; Shukhmina, N.R.; Mel'nikova, N.L.; Nosik, D.N.; Maliushova, V.V.; Andzhaparidze, O.G.
Voprosy Virusologii 38(6): 253-255
1993
ISSN/ISBN: 0507-4088 PMID: 8303883 Document Number: 412264
Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2 anti-env1, and 5 anti-env2). In the immunoblotting assay all the anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them reacted also with p55 protein and with 7 other polypeptides. Another anti-gag Mab cross-reacted with the antigen of subpopulation of human peripheral blood lymphocytes. The third one interacted with the antigen of both HIV-1 and HIV-2. All the 10 Mabs interacted with natural HIV antigens and can be used for identification and differentiation of HIV-1 and HIV-2.