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Preparation and application of monoclonal antibodies to recombinant human IFN alpha

Wu, M.; Ai, Y.; Ren, R.; Liang, Y.; Li, J.; Song, W.; Yang, D.; Sun, J.; Cheng, Y.

Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 16(3): 261-263

2002

ISSN/ISBN: 1003-9279
PMID: 12665935
Document Number: 549635
To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha). Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique. All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%. The highest purification efficiency was obtained by using 4G10 column.

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