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Preparation and characterization of monoclonal antibodies to human apolipoprotein E

Shuvaev, V.V.; Ianushevskaia, E.V.; Dergunov, A.D.

Biulleten' Eksperimental'noi Biologii i Meditsiny 112(8): 179-181

1991

ISSN/ISBN: 0365-9615
PMID: 1786384
Document Number: 371555
Monoclonal antibodies to human apolipoprotein E (apoE) were prepared and characterized. Antibodies of 3D12F11 clone were shown to be specific to apoE and belong to IgG1 subclass. Dissociation constant of antigen-antibody complex in solution was determined as 3.5 .+-. 0.5 nM. The antibodies interacted neither heparin- nor lipid-binding sites of human apoE molecule. The obtained antibodies may be useful for metabolic and structural investigations of apoE as well as for clinical studies.

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