Production and characterization of monoclonal antibodies against a human ovarian teratocarcinoma cell line
Lemieux, P.; Pagé, M.
Anticancer Research 14(6b): 2709-2715
1994
ISSN/ISBN: 0250-7005 PMID: 7872706 Document Number: 437593
Nine monoclonal antibodies (MAbs) of a total of 125 clones selected by an enzyme immunoassy (ELISA) were produced against a human ovarian teratocarcinoma cell line. Within the panel, one antibody (4E3) has shown a close reactivity to OC-125. However, the antigen recognized by 4E3 was found to be different from the CA-125 antigen. The cell surface antigen recognized by 4E3 has a molecular weight over 2 times 10-6 Da and is highly glycosylated as shown by the periodic acid/Schiff reagent.
Document emailed within 1 workday
Related Documents
Smirnov, I.V.; Gryazeva, I.V.; Samoilovich, M.P.; Terekhina, L.A.; Pinevich, A.A.; Krylova, A.A.; Krutetskaia, I.Y.; Nikolsky, N.N.; Klimovich, V.B. 2015: Production and Characterization of the Panel of Monoclonal Antibodies against Human Endoglin Tsitologiia 57(7): 499-508Molthoff, C.F.; Boven, E.; Calame, J.J.; van Beek, P.J.; Pinedo, H.M. 1989: Characterization of human ovarian cancer lines with a panel of monoclonal antibodies (MoAbs) Strahlentherapie und Onkologie: Organ der Deutschen Rontgengesellschaft . 165(7): 494-495
Woodhouse, C.S.; Morgan, A.C. 1989: Murine monoclonal IgG3 antibodies to human colorectal tumor-associated antigens: production and characterization of antibodies active in both antibody-dependent cellular cytotoxicity and complement-mediated cytolysis Cancer Research 49(10): 2766-2772
Young, D.A.; Prout, G.R.; Lin, C.W. 1985: Production and characterization of mouse monoclonal antibodies to human bladder tumor-associated antigens Cancer Research 45(9): 4439-4446
Yoshimura, T.; Takeya, M.; Takahashi, K.; Kuratsu, J.; Leonard, E.J. 1991: Production and characterization of mouse monoclonal antibodies against human monocyte chemoattractant protein-1 Journal of Immunology 147(7): 2229-2233
Böttger, V.; Hering, S.; Jantscheff, P.; Micheel, B. 1985: Monoclonal antibodies against the human leukemia cell line K 562 Archiv für Geschwulstforschung 55(1): 1-8
Sugimoto, T.; Tatsumi, E.; Takeda, K.; Minato, K.; Sagawa, K.; Minowada, J. 1984: Modulation of cell surface antigens induced by 12-O-tetradecanoyl-phorbol 13-acetate in two myeloblastic cell lines, a promyelocytic cell line, and a monoblastic cell line: detection with five monoclonal antibodies Journal of the National Cancer Institute 72(4): 923-928
Briers, T.W.; Stroobants, P.; Vandeputte, T.M.; Nouwen, E.J.; Conraads, M.V.; Eestermans, G.; Van Bockstaele, D.; De Broe, M.E. 1989: Establishment and characterization of a human ovarian neoplastic cell line, DO-s Cancer Research 49(18): 5153-5161
Uehara, S.; Soh, K.; Hoshiai, H.; Yajima, A.; Suzuki, M.; Abe, H. 1983: Establishment and characterization of human ovarian endometrioid carcinoma cell line Nihon Sanka Fujinka Gakkai Zasshi 35(1): 19-26
Nozawa, S.; Sakayori, M.; Takayama, Y.; Tsukazaki, K.; Aoki, D.; Iizuka, R.; Mukai, M.; Inaba, N. 1987: Production of monoclonal antibody against a newly established human endometrial cancer cell line SNG-Ii Nihon Sanka Fujinka Gakkai Zasshi 39(4): 559-566
Pancino, G.; Charpin, C.; Osinaga, E.; Betaille, B.; Le Roy, M.; Calvo, F.; Roseto, A. 1990: Characterization and distribution in human tissues of a glycoproteic antigen defined by monoclonal antibody 1BE12 raised against the human breast cancer cell line T47D Cancer Research 50(22): 7333-7342
Wray, L.K.; Harris, H. 1983: Monoclonal antibodies to an ectopically expressed alkaline phosphatase in a human malignant cell line Cancer Research 43(2): 758-762
Poláková, K.; Chorváth, B.; Sedlák, J.; Duraj, J.; Matoska, J.; Karpatová, M. 1985: Monoclonal antibodies against MHC class Ii antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line Neoplasma 32(6): 641-648
Ishiwata, I.; Ishiwata, C.; Soma, M.; Tomita, K.; Nozawa, S.; Ishikawa, H. 1985: Establishment and characterization of HUOT, a human ovarian malignant teratoma cell line producing alpha-fetoprotein Journal of the National Cancer Institute 75(3): 411-422
Ruiz van Haperen, V.W.; Veerman, G.; Eriksson, S.; Boven, E.; Stegmann, A.P.; Hermsen, M.; Vermorken, J.B.; Pinedo, H.M.; Peters, G.J. 1994: Development and molecular characterization of a 2',2'-difluorodeoxycytidine-resistant variant of the human ovarian carcinoma cell line A2780 Cancer Research 54(15): 4138-4143
Ohi, S.; Niimi, S.; Okada, N.; Yamada, K.; Tachibana, T.; Hashimoto, H.; Nakajima, M.; Yasuda, M.; Tanaka, T.; Sato, K.; Ishikawa, H. 2004: Establishment and characterization of a human ovarian small cell carcinoma, hypercalcemic type, cell line (OS-1) secreting PTH, PthrP and ACTH--special reference to the susceptibility of anti-cancer drugs Human Cell 17(4): 203-209
Fisher, A.G.; Bunce, C.M.; Toksoz, D.; Stone, P.C.; Brown, G. 1982: Studies of human myeloid antigens using monoclonal antibodies and variant lines from the promyeloid cell line HL60 Clinical and Experimental Immunology 50(2): 374-381
Ho, J.J.; Chung, Y.S.; Fujimoto, Y.; Bi, N.; Ryan, W.; Yuan, S.Z.; Byrd, J.C.; Kim, Y.S. 1988: Mucin-like antigens in a human pancreatic cancer cell line identified by murine monoclonal antibodies SPan-1 and YPan-1 Cancer Research 48(14): 3924-3931
Bhan, A.K.; Mihm, M.C.; Dvorak, H.F. 1982: T cell subsets in allograft rejection. in situ characterization of T cell subsets in human skin allografts by the use of monoclonal antibodies Journal of Immunology 129(4): 1578-1583
Cooper, C.S.; Blair, D.G.; Oskarsson, M.K.; Tainsky, M.A.; Eader, L.A.; Vande Woude, G.F. 1984: Characterization of human transforming genes from chemically transformed, teratocarcinoma, and pancreatic carcinoma cell lines Cancer Research 44(1): 1-10