Development, characterization and potential clinical use of monoclonal antibodies against human alphafoetoprotein
Chiplunkar, S.V.; Amin, K.M.; Gangal, S.G.
Indian Journal of Medical Research 90: 1-8
1989
ISSN/ISBN: 0019-5340 PMID: 2470671 Document Number: 335044
Four anti-alphafoetoprotein (AFP) monoclonal antibodies (MAb) were raised in the laboratory and characterized using ELISA and immunodot assays. The affinity constants of the MAbs, analysed by scatchard plots, ranged from 3.1 X 10(8) to 2.15 X 10(9) M/l. Epitope analysis using competition RIA indicated that MAb 5E2D7 and 5E2E3 recognize different epitopes on AFP. This combination was used to set up a two site sandwich ELISA with HRPO conjugated 5E2D7. AFP values in sera of hepatocellular carcinoma patients and pregnant women were quantitated using sandwich ELISA. The anti-AFP MAbs showed strong reactivity when tested on hepatoma tissue sections using immunoperoxidase technique.