Modification of latrotoxin channels under the effect of pronase and antibodies to latrotoxin
Chanturiia, A.N.; Shaturskiĭ, O.Ia.; Nikolaenko, A.N.; Lishko, V.K.
Ukrainskii Biokhimicheskii Zhurnal 61(2): 85-89
1989
ISSN/ISBN: 0201-8470 PMID: 2471341 Document Number: 329978
The action of pronase and latrotoxin antibodies on .alpha.-latrotoxin channels in lipid bilayers has been investigated. Proteolysis of the protruding portions of the inserted channels changed slightly its conductivity and selectivity. Latrotoxin antibodies added to either side of the membrane decreased channel conductivity. The effect depends on the membrane potential. It is suggested that the channel conformation depends on the transmembrane potential and selective centre localized in the inner region of a bilayer is insensitive to proteolysis of protruding segments.