Characterisation of the first monoclonal antibody against the pronase resistant core of the Alzheimer PHF
Novak, M.; Wischik, C.M.; Edwards, P.; Pannell, R.; Milstein, C.
Progress in Clinical and Biological Research 317: 755-761
1989
ISSN/ISBN: 0361-7742 PMID: 2602435 Document Number: 327137
A monoclonal antibody, NOAL 6.423 has been produced which unequivocally identifies a protein which is a constituent of the pronase resistant core of the Alzheimer PHF. Although the site recognised by this antibody is contained somewhere within the highly conserved triple repeat region of the tau molecule, no experiments have succeeded so far in demonstrating NOAL 6.423 reactivity in any brain proteins other than those derived from PHFs. A site which can be recognised by NOAL 6.423 cannot be generated in preparations of normal porcine tau by simple proteolytic cleavage with pronase. Likewise reactivity with the PHF peptide recognised by NOAL 6.423 is not altered by alkaline phosphatase. The unusual properties of NOAL 6.423 are consistent with the possibility that its recognition site is not determined solely by the primary structure of the conserved region of the tau molecule.