A monoclonal antibody that defines p24, a core protein of adult T-cell leukemia virus, and its precursor
Fujii, M.; Sugamura, K.; Hinuma, Y.
Gan 75(7): 595-602
1984
ISSN/ISBN: 0016-450X PMID: 6088349 Document Number: 240727
A mouse hybridoma cell line, H15, produced monoclonal antibody reacting with all the adult T-cell leukemia (ATL) virus (ATLV)-bearing cell lines but none of the ATLV-negative cell lines tested. Binding of H15 antibody to ATLV-bearing cell surfaces was specifically blocked by anti-ATLV positive human sera. Radioimmunoprecipitation analyses revealed that the antigen detected by H15 antibody was p24, a core protein of ATLV. Pulse-chase experiments using H15 antibody led to the identification of a protein, p53, which could be a precursor of p24.
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