Splenic B cells from CBA/N mice acquire responsiveness to anti-immunoglobulin after a brief treatment with pronase
Ramanadham, M.; Gollapudi, S.V.; Kern, M.
Journal of Immunology 134(1): 4-6
1985
ISSN/ISBN: 0022-1767 PMID: 3871111 Document Number: 256558
Although splenic B cells of CBA/N mice do not synthesize DNA in response to anti-mouse IgM (mu-chain specific), the cells respond readily to Sepharose linked anti-mu. Subsequent to a brief treatment with pronase, CBA/N splenocytes exhibited anti-mu-mediated DNA synthesis at 40 to 100% of the DNA synthetic capacity detected with Sepharose linked anti-mu. Furthermore, spleen cell populations treated with anti-Thy-1.2 and complement or populations purified on anti-immunoglobulin-coated Petri plates (greater than 90% surface immunoglobulin positive) acquired responsiveness to anti-mu after pronase treatment.