Interaction of the Latrodectus tredecimguttatus spider venom with liposomes
Trikash, I.O.; Lishko, V.K.
Ukrainskii Biokhimicheskii Zhurnal 57(3): 7-12
1985
ISSN/ISBN: 0201-8470 PMID: 2411041 Document Number: 254826
It is established that interaction of Latrodectus tredecimguttatus venom with bilayer phospholipid membranes of liposomes results in formation of ion channels. Liposome permeability variations are estimated by input and output of 86Rb+ and 45Ca2+ isotopes. It is shown that LaCl3 in 0.5 mM concentration decreases considerably both input and output of Rb+ in proteoliposomes and it may be used as an inhibitor of latrotoxin channels in experiments with phospholipid vesicles. When studying electrophoretic spectrum of proteins bound with liposomes it is shown that latrotoxin (a polypeptide) with molecular mass 130 kDa predominates in proteoliposomes.