Determination of unchanged clobazam in plasma by gas-liquid chromatography
Marliac, Y.; Barazi, S.
Annales Pharmaceutiques Francaises 47(4): 213-220
1989
ISSN/ISBN: 0003-4509 PMID: 2576905 Document Number: 333894
A method has been developed for gas-chromatographic determination of clobazam in human plasma using a nitrogen-selective detector. The unchanged drug was extracted from the plasma at pH 9 by benzene. The method used methyl-1-clonazepam as internal standard. Calibration graphs were linear in the range of 20 to 10,000 ng/ml. The recovery of the compound was 102.29%. The sensitivity of the method was adequate for determination of the drug at toxic concentrations and at therapeutic doses. It was a simple, rapid, reproductible method for determination of clobazam and most of the commercialized benzodiazepines. The method has been tested in a few number of clinicals cases. No interferences occurred in plasma from patients treated with various drugs.