Determination of plasma tocopherols using high performance liquid chromatography
Heng, E.L.; Klein, D.; Debry, G.
International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 53(3): 280-286
1983
ISSN/ISBN: 0300-9831 PMID: 6629667 Document Number: 215859
The authors describe an analytical method for the determination of plasma vitamin E by high performance liquid chromatography. 100 microliters of plasma are added to en ethanol-hexane mixture in presence of ascorbic acid. The hexan phase is concentrated and 50 microliters are injected directly into the chromatography in a silica gel column. The detector is set at 280 nm. The very low quantity of plasma required for the determination makes this method very useful in neonatal medicine.