Determination of ketoprofen in plasma and urine by high-performance liquid chromatography
Jefferies, T.M.; Thomas, W.O.; Parfitt, R.T.
Journal of Chromatography 162(1): 122-124
1979
ISSN/ISBN: 0021-9673 PMID: 310820 Document Number: 144891
The determination of the non-steroidal and anti-inflammatory agent ketoprofen, 2-(3-benzoylphenyl)propionic acid, in plasma and urine is necessary to study its absorption and excretion in patients with rheumatoid arthritis. Previously described methods for the analysis of ketoprofen are time-consuming and lack sensitivity. High-performance liquid chromatography requires minimal sample preparation time and can determine a minimum quantity of 2.5 ng ketoprofen in an analytical time of 4 min. For both urine and plasma samples, the proposed method is accurate, rapid, produces excellent baseline stability and the columns may be used indefinitely.