Determination of synthetic hormones in animal urine by high-performance liquid chromatography/mass spectrometry
Rúbies, A.; Cabrera, A.; Centrich, F.
Journal - Association of Official Analytical Chemists 90(2): 626-632
2007
ISSN/ISBN: 0004-5756 PMID: 17474533 Document Number: 610546
A method was developed for the extraction of stanozolol, taleranol, zeranol, hexestrol, dienestrol, ethynylestradiol, diethylstilbestrol, and trenbolone from animal urine. The analytes were extracted from the matrix by enzymatic hydrolysis, solid-phase extraction, and liquid-liquid extraction. Detection and quantitation were performed on a high-performance liquid chromatography system coupled to a triple quadrupole mass spectrometer. The identification criteria met the European Union regulations. Validation of this method established a decision limit between 0.2 and 0.9 og/L and a detection capability between 0.3 and 1.0 og/L, depending on the analyte.