Determination of beta-agonist residues in bovine urine using liquid chromatography-tandem mass spectrometry
Dickson, L.C.; MacNeil, J.D.; Lee, S.; Fesser, A.C.E.
Journal - Association of Official Analytical Chemists 88(1): 46-56
2005
ISSN/ISBN: 0004-5756 PMID: 15759725 Document Number: 593891
A multiresidue method was developed and validated to screen bovine urine samples for 10 beta-2-adrenergic agonistic drugs-brombuterol, cimaterol, clenbuterol, clenpenterol, isoxsuprine, mabuterol, ractopamine, ritodrine, salbutamol, and tulobuterol-at the 2 microgram/L level. The method is also quantitative in the range of 1 to 4 microgram/L for all analytes except salbutamol. The procedure uses enzymatic digestion, liquid-liquid extraction, and cleanup on solid-phase extraction columns, followed by detection using a liquid chromatograph-tandem quadrupole mass spectrometer operated in the positive-ion atmospheric pressure chemical ionization multiple-reaction monitoring mode. Method validation included assessment of recoveries, repeatabilities, linearity of responses, decision limits, and detection capabilities. Overall average recoveries ranged from 70-91%; recoveries were generally lower for salbutamol. The decision limits ranged from 0.4-1.0 microgram/L, and detection capabilities from 0.6-1.7 microgram/L.