Determination of risperidone in human plasma by liquid chromatography-tandem mass spectrometry
Duan, X-tao.; Chen, X-yan.; Zhang, Y-fan.; Zhong, D-fang.
Yao Xue Xue Bao 41(7): 684-688
2006
ISSN/ISBN: 0513-4870 PMID: 17007366 Document Number: 597653
Aim To develop and validate a liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the determination of risperidone in human plasma. Methods Risperidone and the internal standard, diphenhydramine, were isolated from plasma by liquid-liquid extraction with etherdichloromethane (3: 2, nu/nu), then chromatographed on a Zorbax Extend-C,, column ( 150 mm x 4.6 mm ID, 5 mu m) using a mobile phase consisted of acetonitrile-water-formic acid (40: 60: 0.5, nu/nu), at a flow rate of 0.7 mL (.) min(-1). A Finnigan TSQ tandem mass spectrometer equipped with atmospheric pressure chemical ionization source was used as detector and was operated in the positive ion mode. Selected reaction monitoring (SRM) using the precursor product ion combinations of m/z 411 -> 191 and m/z 256 167 were used to quantify risperidone and diphenhydramine ( IS), respectively. Results The linear concentration range of the calibration curve for risperidone was 0.025 - 50 mu g (.) L-1. The lower limit of quantification was 0.025 mu g (.) L-1. The intra- and inter-day relative standard deviation (RSD) across three validation running over the entire concentration range was less than 7.1 %. The accuracy was within 3.8%. Each sample was chromatographed within 2.7 min. Conclusion The method was proved to be rapid, sensitive and suitable for pharmacokinetic investigations of risperidone.