Determination of nitroimidazole residues in porcine urine by liquid chromatography/tandem mass spectrometry
Xia, X.; Li, X.; Shen, J.; Zhang, S.; Ding, S.; Jiang, H.
Journal - Association of Official Analytical Chemists 89(4): 1116-1119
2006
ISSN/ISBN: 0004-5756 PMID: 16915853 Document Number: 603774
A reliable and sensitive method was developed for determination of nitroimidazoles in porcine urine. Sample preparation involves an extraction with ethyl acetate, followed by a solid-phase extraction cleanup step. The final extract is injected into the liquid chromatography/tandem mass spectrometry system in the positive-ion electrospray ionization mode. A C8 column with water and acetonitrile as the mobile phase is used for chromatographic separation under gradient conditions. Overall average recoveries ranged from 83 to 107%. The limits of detection ranged from 0.03 to 0.05 ng/mL, and the limits of quantitation, from 0.1 to 0.2 ng/mL.