Simultaneous determination of 11 phthalate metabolites in urine by liquid chromatography tandem mass spectrometry
Hu, X.; Zhang, M.; Xu, X.; Ding, C.; Jin, Y.; Lin, S.
Wei Sheng Yan Jiu 41(1): 109-112
2012
ISSN/ISBN: 1000-8020 PMID: 22443069 Document Number: 662488
To develop a method for determining 11 kinds of phthalate metabolites in urine simultaneously. The phthalate metabolites in human urine samples were hydrolyzed by glucuronidase, and then were concentrated by solid-phase extraction and eluted by methanol/acetonitrile (1 : 1, V/V). After chromatographic separation on phenyl column, the analytes were analyzed qualitatively and quantitatively under MRM mode. The limits of detection (LOD) for the eleven kinds of phthalate metabolites were in the range of 0.13 - 2.27 ng/ml. The mean recoveries at the two spiked levels were 67% - 108%, with the relative standard deviation (RSD) of less than 12%. The method can be used to determine the residues of eleven kinds of phthalate metabolites in urine accurately and sensitively.