Quantitative determination of 1,3-bis (tetrahydro-2- furanyl) -5-fluoro-2,4-pyrimidinedione and its metabolites in plasma by high-pressure liquid chromatography and gas chromatography-mass fragmentography
Marunaka, T.; Umeno, Y.
Journal of Chromatography 157: 321-330
1978
ISSN/ISBN: 0021-9673 PMID: 701444 Document Number: 138045
1,3-Bis(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione was developed clinically as an antitumor agent. A high-pressure liquid chromatographic method was developed with which it could be measured in plasma with a sensitivity of 0.050 .mu.g/ml. Two of its metabolites, 1-(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione and 3-(tetrahydro-2-furanyl)-5-fluoro-2,4-pyrimidinedione, could be determined at the same time with a sensitivity of 0.025 .mu.g/ml. A gas chromatographic-mass fragmentographic method was developed for the specific determination of the 3rd metabolite, 5-fluoro-2,4-pyrimidinedione, as its silylated derivative with a sensitivity of 0.001 .mu.g/ml. The precision and sensitivity of the assay appear to be satisfactory for determination of the plasma level fo the drug.