Class-specific and polyvalent enzyme-linked immunosorbent assays for detection of antibodies to Borrelia burgdorferi in equids
Magnarelli, L.A.; Anderson, J.F.
Journal of the American Veterinary Medical Association 195(10): 1365-1368
1989
ISSN/ISBN: 0003-1488 PMID: 2684937 Document Number: 332268
Class-specific and polyvalent ELISAs were developed to detect IgM antibody or total immunoglobulins to B. burgdorferi in equine sera. Analyses of 122 serum specimens, collected during 1985 from horses and ponies in tick-infested areas of Connecticut, revealed IgM antibody in 41 (34%) samples; titration end points ranged from 1:160 to 1:2560. In polyvalent ELISA, 73 (16%) of 454 serum samples contained IgM and/or IgG antibody. Seropositivity was highest (32%) for blood samples collected during May. Both ELISA procedures had comparable sensitivities.