Detection of brucella specific protein-A reactive antibodies in buffaloes by dot-enzyme-linked immunosorbent assay
Chand, P.; Batra, H.V.; Sadana, J.R.
Veterinary Record 122(7): 162-163
1988
ISSN/ISBN: 0042-4900 PMID: 3131948 Document Number: 317349
A dot-enzyme-linked immunosorbent assay (dot-ELISA) was performed using antigen-coated pre-blocked dipsticks prepared with 300 ng sonicated antigen (Brucella abortus S99). They were incubated in serum at 37 degrees C and then in protein-A-horse radish peroxidase conjugate before immersion in substrate solution. A positive reaction was indicated by the development of a brown dot within one minute. When serum samples from 16 aborted buffaloes were tested by a serum-agglutination test (SAT), a complement fixation test (CFT) and the dot-ELISA, 5 buffaloes were positive by SAT and CFT and had high dot-ELISA titres. B. abortus biotype 3 was isolated from the fetus of 2 of these buffaloes. When 68 serum samples from apparently normal in-contact buffaloes were tested 10 had SAT titres (