Enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified P26 viral protein
Shen, D.T.; Gorham, J.R.; McGuire, T.C.
American Journal of Veterinary Research 45(8): 1542-1543
1984
ISSN/ISBN: 0002-9645 PMID: 6089620 Document Number: 236281
An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of equine infectious anemia (EIA) antibody in horse sera. Purified P26 viral protein was the antigen; alkaline phosphatase linked to rabbit anti-horse immunoglobulin G was the conjugate. The ELISA detected EIA antibodies in horse sera as early as 11 to 14 days after experimental inoculations. There was full agreement between the results of ELISA and the agar-gel immunodiffusion tests on EIA proficiency test sera. The ELISA readily detected EIA antibody in horse sera that had weak positive reactions on agar-gel immunodiffusion.