Rapid detection of canine parvovirus in feces using monoclonal antibodies and enzyme-linked immunosorbent assay
Mildbrand, M.M.; Teramoto, Y.A.; Collins, J.K.; Mathys, A.; Winston, S.
American Journal of Veterinary Research 45(11): 2281-2284
1984
ISSN/ISBN: 0002-9645 PMID: 6098198 Document Number: 228556
Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay [ELISA] for the detection of canine parvovirus (CPV) antigen in fecal samples. The assay was specific for the hemagglutinating protein of CPV and detected as little as 1.5 ng of virus within a 15 min incubation period. The use of monoclonal antibodies against 2 epitopes on the CPV antigen permitted the simultaneous addition of test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation. Clinical studies revealed > 95% correlation between ELISA results and hemagglutination titers.