Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus

Fiscus, S.A.; Mildbrand, M.M.; Gordon, J.C.; Teramoto, Y.A.; Winston, S.; Fiscus, S.A; Mildbrand, M.M; Gordon, J.C; Teramoto, Y.A; Winston, S

Home

>

Node

>

Subnode

Rapid enzyme-linked immunosorbent assay for detecting antibodies to canine parvovirus

Fiscus, S.A.; Mildbrand, M.M.; Gordon, J.C.; Teramoto, Y.A.; Winston, S.

American Journal of Veterinary Research 46(4): 859-863

1985

ISSN/ISBN: 0002-9645

PMID: 2990264

Document Number: 264953

A rapid screening assay for determining antibodies to canine parvovirus in dog serum using monoclonal antibodies and enzyme-linked immunosorbent assay (ELISA) was developed. The ELISA could be read visually, and the results correlated well with serum neutralization (SN) and haemagglutination inhibition (HI) titres. Serum with SN 1:4 or HI 1:10 had an 87.9% correlation with ELISA, and serum with SN 1:64 or HI 1:80 had a 94.4% correlation. The assay took only 10 to 15 minutes to perform and did not require specialized equipment. The ELISA should be useful in monitoring dogs for the presence of maternal antibodies against parvovirus and for determining seroconversion after vaccination.