Blood volume in inbred strain BALB/c, CBA/J and C57BL/10 mice determined by means of 59Fe-labelled red cells and 59Fe bound to transferrin
Vácha, J.
Physiologia Bohemoslovaca 24(5): 413-419
1975
ISSN/ISBN: 0369-9463 PMID: 127187 Document Number: 92249
Circulating red blood cell (RBC) and plasma volumes were measured in male inbred mice, strains BALB/C, CBA/J and C57BL/10, by simultaneous use of 59Fe-labelled RBC dilution and dilution of 59Fe bound to transferrin. The whole blood volume values derived from the venous haematocrit and plasma volume were about double those calculated from the venous haematocrit and circulating RBC volume. Comparison of the two methods explains the significant differences in different studies of blood volume in mice and shows that correct values can be found only by parallel measurements of RBC and plasma volume by separate methods, or by correcting the venous haematocrit to whole body haematocrit. Combination of the labelled RBC method and the 59Fe-transferrin method showed the blood volume values in those strains of mice to be 10.35, 7.32 and 7.94 ml/g bodyweight, respectively. The ratio of whole body to venous haematocrit was 57.3, 68.0 and 69.5%. There were significant interstrain differences in RBC, plasma and blood volume and in the venous and whole body haematocrit and their ratio.