Coexpression of genes located in the 111.35-116.16 Mb of chromosome 13 in mice with different predisposition to catalepsy
Siniakova, N.A.; Kulikov, A.V.
Molekuliarnaia Biologiia 48(5): 733-741
2014
ISSN/ISBN: 0026-8984 PMID: 25842858 Document Number: 678885
Catalepsy is a pathologic behavior which is usually associated with a dysfunction of striatal pallidal pathway and which can be caused by different mechanisms. It was showned previously that hereditary catalepsy is linked with the distal 111.35-116.16 Mb of chromosome 13. We investigated the level of mRNA of 42 genes located in this fragment in two brain regions which are concerned with catalepsy-striatum and substantia nigra in catalepsy-resistant AKR mice strain, in cataleptic CBA mice strain and in congenic cataleptic AKR.CBA-D13Mit76 (D13) mice strain which were created by transferring of this fragment from CBA in AKR genome. We showed congenic D13 mice vary from AKR in level of mRNA of 2 genes (Ndufs4 and Ppap2a genes) in striatum and 10 genes (Esm1, Fst, Gm10735, Gm15322, Gm15323, Gm15324, Gm15325, Il6st, II31ra, Itga1) in sibstantia nigra. The level of mRNA of Mcidas gene is reduced in both brain regions in D13 compared to AKR. Gene expression of Hspb3 n Mocs2, which codes heat shock protein and, molybdenum cofactor synthesis, respectively, in substantia nigra is reduced in cataleptic CBA and D13 mice compared to catalepsy-resistant AKR mice. These genes can be considered as the most likely genes candidate of catalepsy. The revealed genes coexpression shows that there is a difficult genes network, which regulates hereditary catalepsy.