Determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography
Zhang, M.S.; Huang, J.X.
Se Pu 19(5): 470-471
2001
ISSN/ISBN: 1000-8713 PMID: 12545450 Document Number: 538373
A method for the determination of solanesol in the extracts of tobacco leaves by high performance liquid chromatography has been developed. The column used was Shim-pack CLC-SIL(5 microns, 5 mm i.d. x 150 mm) and the UV detector was set at 215 nm. The eluent was a mixture of n-hexane and isopropyl alcohol (98:2, V/V). As the results showed, the linear range was 1 microgram-10 micrograms(r = 0.9997), the average recovery of solanesol was 98.1% and the relative standard deviation was 1.9%. This method is fast, accurate and reproducible.