The biological effects of transforming growth factor-beta on human periodontal ligament fibroblasts
Si, X.; Liu, Z.
Zhonghua Kou Qiang Yi Xue Za Zhi 36(1): 23-26
2001
ISSN/ISBN: 1002-0098 PMID: 11812297 Document Number: 538372
To evaluate the biological effects of transforming growth factor-beta (TGF-beta) on human periodontal ligament fibroblasts (HPDLFs). HPDLFs were primary cultured and examined for the effects of TGF-beta on its proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules. TGF-beta(0.0050-0.1000 mg/L) significantly stimulated the proliferation of HPDLFs. The ALP activity of HPDLFs was elevated evidently by 0.0050 mg/L TGF-beta. TGF-beta (0.0005-0.1000 mg/L) had no effects on OC synthesis and formation of the mineralized nodules of HPDLFs. The effects of TGF-beta on HPDLFs are dose-dependent. TGF-beta can stimulate HPDLFs to express some of osteoblastic phenotype, and it lacks the abilility to promote maturation of the osteogenic phenotype.