The effects of transforming growth factor-beta and insulin on proliferation of human periodontal ligament cells
Wu, Z.; Dong, G.; Wan, L.
Zhonghua Kou Qiang Yi Xue Za Zhi 32(5): 300-302
1997
ISSN/ISBN: 1002-0098 PMID: 11189293 Document Number: 477301
To evaluate biological effects in vitro of transforming growth factor-beta (TGF-beta) and/or insulin on proliferation of the human periodontal ligament (PDL) cells. Cell cultures and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay. TGF-beta at concentrations of 0.1 to 10 micrograms/L and insulin at 10 to 1,000 U/L had significant proliferative effects on human PDL cells (P < 0.01). From the 3rd day, the significant proliferation of PDL cells by TGF-beta or insulin were observed (P < 0.01) and lasted to the 5th day. The effect of combination of TGF-beta with insulin was the most significant one (P < 0.01). TGF-beta and/or insulin may be valuable in promoting the regeneration of periodontal connective tissue.