Determination of amygdalin in hawthorn by high performance liquid chromatography
Lü, W.; Ding, M.
Se Pu 23(5): 496-498
2005
ISSN/ISBN: 1000-8713 PMID: 16350793 Document Number: 586691
A suitable method for extraction of amygdalin from hawthorn has been established. At first, the lipophilic components were removed with petroleum ether by ultrasonic extraction. The amygdalin was then extracted by methanol in a Soxhlet's apparatus. For quantitation, a high performance liquid chromatographic method was developed by using a reversed-phase C18 column, mobile phase of methanol-water (15:85, v/v) and a detection wavelengh of 215 nm. It can be concluded that the content of amygdalin is higher in the seeds than that in the hawthorn powder without the seeds and the yield of amygdalin is higher in the hawthorn pieces than that in the hawthorn powder.