Determination of chlorfluazuron residue in foods by high performance liquid chromatography
Du, L.; Song, J.; Zhang, L.; Liu, H.
Se Pu 29(12): 1240-1243
2011
ISSN/ISBN: 1000-8713 PMID: 22500454 Document Number: 654398
A method for the determination of chlorfluazuron residue in foods by high performance liquid chromatography (HPLC) was developed. The samples were extracted with hexane or acetonitrile. After cleaned up with Florisil-solid phase extraction (SPE), the samples were analyzed by the separation on a C18 chromatographic column with an acetonitrile and water (85 : 15, v/v) mixed solution as the mobile phase and the determination with an ultraviolet detector at 260 nm. The linear range was 0.05 - 2.0 mg/L, the correlation coefficient was 0.999 8 and the limit of quantification (S/N = 10) was 0.05 mg/kg. The recoveries were 82.1% - 102.5% with the relative standard deviations (n = 10) of 3.00% - 6.25% at the three different spiked levels of 0.05, 0.1 and 1 mg/kg. The method is easy, fast, accurate and consuming less sample and organic solvents. It can be applied in the determination of chlorfluazuron in foods.