Studies on chemical structure of polysaccharide from fruit body of Coriolus versicolor
Zhang, J.S.; Han, W.W.; Pan, Y.J.
Yao Xue Xue Bao 36(9): 664-667
2001
ISSN/ISBN: 0513-4870 PMID: 12580103 Document Number: 537442
To study the structure of polysaccharides from Coriolus versicolor. By chromatography a homogeneous polysaccharide B-1-3 was obtained. The structure of B-1-3 was elucidated using the method of total acid hydrolysis, methylation, and 1D and 2D NMR. B-1-3 was found to be mainly composed of Glc by total acid hydrolysis. Its molecular weight was estimated to be 3.16 x 10(5) by HPLC method. Methylation analysis and 1H and 13CNMR indicated the main chain of B-1-3 consisted of beta-D-1,4-Glc and beta-D-1,3-Glc, and branch chains were situated, beta-D-1,3,6-Glc and beta-D-1,4,6-Glc. 1H and 13CNMR also showed that beta-D-Glc-1-->3-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc and beta-D-Glc-1-->4-beta-D-Glc-beta-D-Glc-1-->4-beta-D-Glc fragments existed in B-1-3. B-1-3 is a glucan which main chain was composed of beta-D-1,4-Glc and beta-D-1,3-Glc beta-D-1,6-Glc.