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Optimization of a method for purifying antibodies for immunofluorescent diagnosis of influenza

Mamiushichev, V.B.; Potapenko, L.B.; Dement'ev, A.A.; Sokolov, I.N.

Voprosy Virusologii 46(1): 44-47

2001

ISSN/ISBN: 0507-4088
PMID: 11233289
Document Number: 535461
Efficiency and diagnostic adequacy of immunoglobulin isolation from rabbit immune serum by reiterated salting out with ammonium sulfate and subsequent separation of DEAE sephadex A-50 was evaluated. Electrophoresis and immunoelectrophoresis in agar gel demonstrated that the resultant immunoglobulin fraction is much more pure than after one salting out. It contains nothing more than antibodies to influenza virus taken for immunization, and after binding to the stain shows high fluorescent activity and induces but a moderate background nonspecific fluorescence in the subsequent preparations. In clinical trials of conjugates based on the new combined method of purification, influenza A or B could be correctly diagnosed in 97-98% cases.

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